Mitosis in focus

Foci of cyclin A2 interact with actin and RhoA in mitosis

Cyclin A2 is a key player in the regulation of the cell cycle. Its degradation in mid-mitosis depends primarily on the ubiquitin-proteasome system (UPS), while autophagy also contributes. However, a fraction of cyclin A2 persists beyond metaphase. In this work, we focus on cyclin A2-rich foci detected in mitosis by high resolution imaging and analyse their movements. We demonstrate that cyclin ...

Multispectral Spatial Characterization: Application to Mitosis Detection in Breast Cancer Histopathology

Accurate detection of mitosis plays a critical role in breast cancer histopathology. Manual detection and counting of mitosis is tedious and subject to considerable interand intra-reader variations. Multispectral imaging is a recent medical imaging technology, proven successful in increasing the segmentation accuracy in other fields. This study aims at improving the accuracy of mitosis detectio...

Modelling chromosome dynamics in mitosis: a historical perspective on models of metaphase and anaphase in eukaryotic cells.

Mitosis is the process by which the genome is segregated to form two identical daughter cells during cell division. The process of cell division is essential to the maintenance of every form of life. However, a detailed quantitative understanding of mitosis has been difficult owing to the complexity of the process. Indeed, it has been long recognized that, because of the complexity of the molec...

Continuation of mitosis after selective laser microbeam destruction of the centriolar region

The centriole regions of prophase PTK2 cells were irradiated with a laser microbeam. Cells continued through mitosis normally. Ultrastructural analysis revealed either an absence of centrioles or severely damaged centrioles at the irradiated poles. Microtubules appeared to focus into pericentriolar cloud material.

Prevention of Acetaminophen-Induced Mitodepression with Myrobalan (Fruit of Terminalia chebula) in Allium cepa Model

Allium cepa bulbs were grown in pure tap water (group I), in seven concentrations of acetaminophen (7.81, 15.62, 31.25, 62.50, 125, 250, 500, and 1000 ppm) in the presence (group II) and absence of myrobalan (fruit of Terminalia chebula) at a fix concentration of 0.10 mg/mL. Parameters of study were mean root length, mitotic index, abnormal mitosis and chromosomal aberrations and morphology of ...

The Kinetochore and Mitosis: Focus on the Regulation and Correction Mechanisms of Chromosome-to-Microtubule Attachments